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在由黄瓜的两个自交系S06与S52杂交产生的F2群体中,应用SRAP(sequence-relatedamplifiedpolymorphism)标记构建遗传连锁图谱,检测控制黄瓜侧枝数(lbn)和侧枝平均长度(lbl)的数量性状座位(QTL).使用筛选出的64个多态性引物组合对F2群体进行分析,得到108个多态性位点.经MAPMAKER/EXP3.0分析(LOD>3.0),获得由92个标记座位组成、覆盖7个连锁群的遗传图谱,总长1164.2cM,标记平均间距12.6cM.应用QTLMapper1.6,各检测到4个控制lbn和lbl的QTL,其中,对lbn贡献率最大的QTL位于第二连锁群的ME11SA4B-ME5EM5区间,其S06基因型具有增效作用;对lbl贡献率最大的QTL位于第二连锁群的DC1OD3-DC1EM14区间,其S06基因型具有增效作用.
The genetic linkage map was constructed by using sequence-related amplifiedional motif (SRAP) markers and the quantitative traits controlling the number of lateral branches (lbn) and the average lateral branches (lbl) of cucumber in the F2 population derived from crossing two inbred lines S06 and S52 of cucumber (QTL) were used to analyze the F2 population using the 64 polymorphic primer combinations, and 108 polymorphic loci were obtained.According to MAPMAKER / EXP3.0 analysis (LOD> 3.0), 92 marker loci , Covering a genetic map of 7 linkage groups with a total length of 1164.2 cM and average marker spacing of 12.6 cM. Four QTLs controlling lbn and lbl were detected using QTLMapper 1.6, of which the QTL with the largest contribution to lbn was located in the second The linkage group of ME11SA4B-ME5EM5 interval, the S06 genotype synergy; the largest contribution to the lbl QTL located in the second linkage group DC1OD3-DC1EM14 interval, the S06 genotype synergy.