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目的:探讨热凝脑膜中动脉对硝酸甘油诱发的偏头痛大鼠静脉血降钙素基因相关肽(CGRP)和P物质(SP)含量的影响。方法:24只健康雌性Wistar大鼠随机分为4组:A组(生理盐水组)为皮下注射生理盐水,B组(硝酸甘油组)为皮下注射硝酸甘油注射液10 mg/kg构建偏头痛动物模型,C组(假手术组)为单纯暴露脑膜中动脉后皮下注射硝酸甘油注射液10 mg/kg,D组(手术组)为热凝脑膜中动脉后皮下注射硝酸甘油注射液10 mg/kg。观察各组大鼠行为学变化及应用放射免疫法(RIA)检测各组大鼠静脉血CGRP和SP含量。结果:A组大鼠偶尔出现挠头现象,D组大鼠与B、C组大鼠相比,挠头及爬笼次数明显减少且频度降低,无耳红现象。4组大鼠静脉血CGRP含量分别为(12.14±4.99)、(57.34±10.27)、(51.40±13.13)和(27.96±8.32)ng/L;SP含量分别为(6.78±2.09)、(20.03±5.74)、(22.33±6.83)和(13.27±2.31)ng/L。B、C及D组大鼠静脉血CGRP和SP含量与A组相比均有增加,差异具有统计学意义,且D组大鼠静脉血CGRP和SP含量低于B和C组大鼠,差异具有统计学意义,但B与C组之间差异无统计学意义(均P>0.05)。结论:热凝大鼠脑膜中动脉可减轻由硝酸甘油诱发的偏头痛症状,这可能是通过抑制CGRP和SP的释放引起的。
Objective: To investigate the effect of thermal subaramanial arteries on the content of calcitonin gene related peptide (CGRP) and substance P (SP) in nitroglycerin-induced migraine rats. Methods: Twenty-four healthy female Wistar rats were randomly divided into 4 groups: group A (saline) injected subcutaneously with normal saline; group B (nitroglycerin) injected subcutaneously with nitroglycerin 10 mg / kg to construct migraine animal The rats in group C (sham operation group) were treated with 10 mg / kg nitroglycerin injection subcutaneously in the middle meningeal artery and group D (operation group) with subcutaneous injection of nitroglycerin injection 10 mg / kg . The behavioral changes of rats in each group were observed and radioimmunoassay (RIA) was used to detect the contents of CGRP and SP in venous blood of rats in each group. Results: Occasionally scratching occurred in rats in group A, while the number of scratching and climbing cage in group D was significantly reduced and the frequency was lower than that in group B and C. The levels of CGRP in the venous blood of the 4 groups were (12.14 ± 4.99), (57.34 ± 10.27) and (51.40 ± 13.13) and (27.96 ± 8.32) ng / L, respectively; the contents of SP were 6.78 ± 2.09 and 20.03 ± 5.74), (22.33 ± 6.83) and (13.27 ± 2.31) ng / L, respectively. The levels of CGRP and SP in venous blood of rats in groups B, C and D increased compared with those in group A, and the difference was statistically significant. The levels of CGRP and SP in venous blood of group D were lower than those in groups B and C With statistical significance, but there was no significant difference between B and C groups (all P> 0.05). CONCLUSIONS: The thermal coagulation of the middle meningeal artery in rats may alleviate migraine symptoms induced by nitroglycerin, possibly by inhibiting the release of CGRP and SP.