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目的:建立测定积雪草配方颗粒剂中积雪草苷的HPLC含量分析方法。方法:采用C18柱,RP-HPLC法,水-乙腈为流动相,梯度洗脱,检测波长205 nm。结果:积雪草苷在0.02-0.51 mg·mL-1范围内,峰面积与浓度呈线性关系,r=0.9999,高、中、低浓度的平均回收率分别为99.6%,101.7%,102.2%;RSD分别为2.9%,1.7%,4.9%(n=3)。结论:该方法简便,结果准确,适用于积雪草配方颗粒剂的质量控制。
Objective: To establish HPLC method for determination of asiaticoside in Centella asiatica granules. METHODS: C18 column, RP-HPLC, water-acetonitrile as mobile phase, gradient elution, detection wavelength at 205 nm. Results: The concentration of asiaticoside in the range of 0.02-0.51 mg·mL-1 showed a linear relationship with the concentration, r=0.9999. The average recoveries of high, medium and low concentrations were 99.6%, 101.7% and 102.2%, respectively. RSDs were 2.9%, 1.7%, and 4.9% (n=3), respectively. Conclusion: This method is simple, accurate, and suitable for the quality control of Centella asiatica granules.