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目的:为临床研究提供腹泻病动物模型。方法:使用致病性大肠杆菌0111菌株,通过腹腔注射和经口灌胃两种途径感染小鼠。结果:经腹腔注射,可诱导小鼠腹泻,大便培养阳性,半数致死量为3.0亿/只,半数致腹泻量为2.4亿/只;经口灌胃30亿/只,小鼠未致端正。结论:经腹腔注射致病性大肠杆菌0111菌株可诱导出小鼠腹泻模型。
Objective: To provide animal models of diarrhea for clinical research. Methods: The pathogenic E. coli 0111 strain was used to infect mice by intraperitoneal injection and oral gavage. Results: After intraperitoneal injection, diarrhea was induced in mice and the stool culture was positive. The median lethal dose was 300 million / bird, half of which caused 240 million / bird flu. Conclusion: The diarrhea model of mice can be induced by intraperitoneal injection of pathogenic E. coli 0111.