一、前言杂交水稻为我国首创。实践证明,它不但有明显的增产优势,而且适应性广,抗逆性强。已大面积的推广,深受农民群众欢迎,并为国外所推崇。众所周知,种子纯度高低,在生产中有着极其重要的意义。常规品种纯度检验,都以田间检验为主,室内检验为辅。然而杂交水稻只利用其杂种一代。因此,田间检验即无意义,必须进行室内检验。关于鉴定杂交水稻三系和F_1的研究,颜启传、谭学庭等曾做了大量工作,在判断种子真实性方面取得了可喜的成绩。但在检验种子纯度方面。仍须探索。我们曾用裂解色谱法及同工酶电泳法鉴别单粒种子, I. Introduction Hybrid rice is the first in our country. Practice has proved that it not only has obvious yield advantage, but also has wide adaptability and strong resistance. Has been a large area of ​​promotion, welcomed by farmers, and respected abroad. It is well known that the purity of seeds is extremely important in production. Purity testing of conventional varieties, are field-based testing, laboratory testing supplemented. However hybrid rice only uses its hybrid generation. Therefore, the field test that is meaningless, we must conduct indoor testing. Regarding the identification of the three lines and F1 in hybrid rice, Yan Qichuan and Tan Xueting have done a great deal of work and made gratifying achievements in judging the authenticity of the seeds. However, in the inspection of seed purity. Still have to explore. We used pyrolysis chromatography and isozyme electrophoresis to identify single seeds,