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本实验观察补充维生素E的大鼠低温暴露后血清GPT、GOT和LDH活性的变化,探索维生素E对低温暴露动物的影响。将体重70～120g雄性大鼠随机分为甲、乙、丙三组,饲以基础饲料,甲组动物每两天腹腔注射维生素E5mg(5mg/ml),5次(10天)后,将甲、丙两组移入低温室(-1±1℃)连续暴露48小时,乙组作为常温对照留在室温下(20±1℃)。低温暴露结束时,立即测定三组动物血清中GPT、GOT和LDH活性。结果是:丙组动物血清中三种酶活性最高,乙组最低,甲组稍高于乙组但明显低于丙组。说明低温暴露动物补充维生素E后血清中三种酶活性稍有改善。
This experiment observed changes in serum GPT, GOT, and LDH activity after vitamin C supplementation in rats exposed to low temperatures, and explored the effects of vitamin E on animals exposed to low temperatures. Male rats weighing 70-120 g were randomly divided into three groups A, B, and C and fed with basal diet. Group A animals received intraperitoneal injection of vitamin E 5 mg (5 mg/ml) every two days. After 5 times (10 days), they were given C and C groups were moved into the low-temperature chamber (-1±1°C) for 48 hours, and Group B was left at room temperature (20±1°C) as a room temperature control. At the end of the low temperature exposure, the serum GPT, GOT, and LDH activity were measured immediately in the three groups of animals. As a result, the activity of the three enzymes in the serum of the group C animals was the highest and the lowest in the group B. The group A was slightly higher than the group B but significantly lower than the group C. It was demonstrated that the activity of three enzymes in serum was slightly improved after low-temperature exposure of animals to vitamin E supplementation.