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目的探讨抗抑郁药物去甲替林对氧糖剥夺损伤皮质神经元的保护作用及其机制。方法采用体外培养的原代大鼠皮层神经元细胞,经不同浓度(1.0、2.5、5.0、10.0μmol/L)的去甲替林预处理后,建立氧糖剥夺模型。应用免疫细胞染色、MTT和台盼蓝染色检测去甲替林对神经元细胞状态、细胞活性、死亡率的影响。应用Western杂交测定去甲替林对线粒体细胞色素c、Smac/Diablo、AIF释放的影响。结果去甲替林能够明显改善氧糖剥夺损伤后神经元的生存状况,提高细胞存活率,并且明显抑制了损伤后神经元线粒体细胞色素c、Smac/Diablo、AIF的释放。结论抗抑郁药物去甲替林通过抑制细胞线粒体细胞凋亡因子(细胞色素c、Smac/Diablo、AIF)释放来保护氧糖剥夺损伤的皮质神经元,为抗抑郁药物去甲替林神经保护作用深入研究奠定了基础。
Objective To investigate the protective effect of antidepressant nortriptyline on oxygen-glucose deprived cortical neurons and its mechanism. Methods Primary rat cortical neurons cultured in vitro were pretreated with nortriptyline at different concentrations (1.0, 2.5, 5.0 and 10.0 μmol / L) to establish an oxygen-glucose deprivation model. The effects of nortriptyline on neuronal cell status, cell viability, and mortality were examined by immunocytochemical staining, MTT and trypan blue staining. The effect of nortriptyline on mitochondrial cytochrome c, Smac / Diablo and AIF release was determined by Western blot. Results The nortriptyline significantly improved neuronal survival after oxygen deprivation injury, increased cell survival, and significantly inhibited the release of mitochondrial cytochrome c, Smac / Diablo and AIF after injury. Conclusion The antidepressant nortriptyline protects cortical neurons damaged by oxygen-glucose deprivation by inhibiting the release of mitochondrial apoptotic factors (cytochrome c, Smac / Diablo, AIF), which is the neuroprotective effect of antidepressant nortriptyline In-depth study laid the foundation.