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目的探讨定量检测血袋保存液中细菌内毒素(BET)的方法。方法用碱性调节剂来消除血袋保存液中枸椽酸盐类抗凝剂对鲎试剂检查的干扰,建立标准曲线,对处理后的血袋保存液用BET-32M细菌内毒素检测系统进行定量检测,同时用凝胶法作对照。结果动态浊度法定量检测细菌内毒素检测数据符合药典规定的限值(5.56EU/mL),并与凝胶法结果一致,检测结果准确、可靠。结论建立的内毒素检测方法可用于血袋保存液的定量检测。
Objective To explore a method for the quantitative determination of bacterial endotoxin (BET) in blood bag preservation solution. Methods Alkaline regulators were used to eliminate the interference of citrate anticoagulants in the blood bag preservative solution and to establish a standard curve. The treated blood bag preservative solution was determined by BET-32M bacterial endotoxin detection system Quantitative detection, while using gel method for control. Results The results of dynamic turbidimetric assay for detection of bacterial endotoxin were in accordance with the limits of Pharmacopoeia (5.56EU / mL). The results were in good agreement with the gel method and the results were accurate and reliable. Conclusion The established method for detecting endotoxin can be used for the quantitative detection of blood bag preservation solution.