近年来,随着分子生物学技术的进展,对HDV的认识日渐深入,本文拟就当前国内外病毒学界对HDV的研究现状综述如下。一、HDV(又称δ因子) 1.HDVRNA HDVRNA的分子生物学研究已取得了重要突破。1986年,Denniston氏等以HDV RNA为模板获得166个碱基对的互补DNA(cDNA)片段,并重组至质粒pBR_(322)中,成功地在大肠杆菌中获得稳定的表达。此后,Wang氏等又先后获得7个cDNA库。它们分别为δ_1(核苷酸个数为567)。δ_2(250),δ_3 In recent years, with the progress of molecular biology technology, the understanding of HDV is getting deeper and deeper. This paper is intended to summarize the current research status of HDV in virology community at home and abroad as follows. First, HDV (also known as δ factor) 1. HDVRNA HDVRNA molecular biology has made important breakthroughs. In 1986, Denniston et al. Obtained a 166-bp complementary DNA (cDNA) fragment using HDV RNA as a template and recombined into the plasmid pBR_ (322) to obtain stable expression in E. coli. Since then, Wang et al have obtained seven cDNA libraries. They are δ_1 (the number of nucleotides is 567). δ_2 (250), δ_3