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应用定量逆转录聚合酶链反应（ＲＴ－ＰＣＲ）对４例多发性硬化症（ＭＳ）脑脊髓液（ＣＳＦ）细胞及末梢血单个核细胞（ＰＢＭＣ）中Ｔ细胞抗原受体（ＴＣＲ）Ｖ_β基因（Ｖ_β１～Ｖ_β２０）表达进行了探讨。结果表明：未经刺激的ＣＳＦ细胞和ＰＢＭＣ表达广谱Ｖ_β基因，经ＴＣＧＦ／ＩＬ－２／ＩＬ－４短期培养的ＣＳＦ细胞则表达限制性Ｖ_β基因，而经同样培养的ＰＢＭＣ各Ｖ_β基因表达的百分率同培养前ＰＢＭＣ相同。说明在ＭＳ疾病的中枢神经系统中存在着对某种抗原的特异性Ｔ细胞应答，为ＭＳ的免疫治疗提供了新的启示。
The expression of T cell antigen receptor (TCR) V_β in cerebrospinal fluid (CSF) and peripheral blood mononuclear cells (PBMC) of 4 patients with multiple sclerosis (MS) was determined by quantitative reverse transcriptase-polymerase chain reaction (V_β1 ~ V_β20) expression were discussed. The results showed that the unstimulated CSF cells and PBMC expressed broad-spectrum V_β gene, while the CSF cells cultured in short-term TCGF / IL-2 / IL-4 expressed restricted V_β gene. However, the expression of V_β gene The percentage is the same as the pre-culture PBMC. Indicating that there is a specific T cell response to certain antigen in the central nervous system of MS disease, which provides a new inspiration for the immunotherapy of MS.