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目的观察高盐饮食对大鼠肾脏骨调素mRNA及其蛋白表达的影响,旨在研究高盐对大鼠肾脏的损伤作用。方法选取10周龄正常盐饮食雄性Sprague-Dawley大鼠48只,随机分为高盐(4%NaCl)或正常盐(0.6%NaCl)饮食组,每组24只。每周测量大鼠鼠尾血压、体质量。于第4、8、12周末每组各处死6只大鼠,测量大鼠肾质量。采用免疫组化方法测定大鼠肾脏OPN表达;荧光定量PCR方法测定大鼠肾脏骨调素mRNA表达。结果两组大鼠血压、体质量及肾质量/体质量比无明显差异(P<0.05),12周后高盐饮食大鼠肾脏骨调素mRNA(0.27±0.16vs0.15±0.13,P<0.05)及其蛋白表达明显升高(0.78±0.15vs0.61±0.11,P<0.01),与正常盐饮食大鼠比较有显著统计学差异。结论高盐饮食摄入增加大鼠肾脏骨调素mRNA及其蛋白表达,表明高盐对大鼠肾脏有一定损伤作用,并且这种损伤作用不依赖于大鼠血压升高。
Objective To observe the effect of high-salt diet on osteopontin mRNA and its protein expression in rat kidney, aiming to study the injury effect of high salt on rat kidney. Methods Forty eight male Sprague-Dawley rats, 10 weeks old, were randomly divided into high salt (4% NaCl) or normal saline (0.6% NaCl) diet, 24 in each. Rat tail blood pressure and body weight were measured weekly. At the end of the 4th, 8th and 12th week, 6 rats were killed in each group, and the kidney mass of rats was measured. Immunohistochemistry was used to determine the expression of OPN in rat kidney, and the mRNA expression of osteopontin in rat kidney was determined by real-time PCR. Results There was no significant difference in blood pressure, body weight and kidney mass / body weight ratio between the two groups (P <0.05). After 12 weeks, the osteocalcin mRNA in kidney of high-salt diet rats (0.27 ± 0.16 vs 0.15 ± 0.13, P < 0.05) and its protein expression was significantly increased (0.78 ± 0.15vs0.61 ± 0.11, P <0.01), compared with normal saline diet rats were statistically significant differences. Conclusion High salt intake increases osteopontin mRNA and protein expression in rat kidney, indicating that high salt has a certain damage on rat kidney, and this injury does not depend on the increase of blood pressure in rats.