用盐析法、阴、阳离子交换柱层析及凝胶过滤法，从人前列腺组织中分离纯化前列腺特异性抗原（ＰＳＡ），经ＳＤＳ－ＰＡＧＥ显示其分子最为３４ＫＤ。以该抗原常规兔疫家兔，获得抗ＰＳＡ多克隆抗体，此抗体经进一步纯化后，以免疫印迹技术证实它具有ＰＳＡ＿（３４）特异性。ＡＢＣ法显示该抗体对正常、肥大及癌变的前列腺腺上皮呈阳性反应，而对其它正常及肿瘤组织呈阴性反应。用ＥＬＩＳＡ夹心法分析该抗体血清学特异性，结果表明前列腺癌患者血清ＰＳＡ的含量非常显著地高于对照组。 The prostate-specific antigen (PSA) was isolated and purified from human prostate tissue by salting-out method, anion exchange chromatography, cation exchange chromatography and gel filtration. SDS-PAGE showed that the molecule was 34KD. Anti-PSA polyclonal antibody was obtained by conventional rabbit immunization with this antigen. The antibody was further purified and confirmed to have PSA 34 specificity by immunoblotting. ABC method showed that the antibody was positive for normal, hypertrophic and cancerous prostatic gland epithelium, but negative for other normal and tumor tissues. Serological specificity of the antibody was analyzed by ELISA sandwich method. The results showed that the serum PSA level of prostate cancer patients was significantly higher than that of the control group.