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目的提高盾叶薯蓣药材的品质。方法采用高效液相色谱法,对组织培养条件下诱导获得的盾叶薯蓣四倍体株系试管苗薯蓣皂苷元的量和部分株系一年生田间苗薯蓣皂苷元的量进行了分析,并对部分株系一年生田间苗农艺性状进行了鉴定。结果经诱导获得的四倍体株系试管苗与对照相比薯蓣皂苷元的量有较大差异;而且四倍体株系试管苗与一年生苗薯蓣皂苷元的量有较强的相关性,大部分四倍体株系表现出典型的多倍体性状。结论在组织培养的基础上,利用多倍体育种来改善盾叶薯蓣的品质是可行的。
Objective To improve the quality of Dioscorea zingiberensis. Methods The amount of diosgenin in tetraploid strains of Dioscorea zingiberensis and the amount of diosgenin in annual seedlings of some strains induced by tissue culture were analyzed by high performance liquid chromatography (HPLC) The annual farmland seedling agronomic traits were identified. Results The quantity of diosgenin in the tetraploid plantlets induced by the induction was significantly different from that of the control. Moreover, the tetraploid plantlets showed strong correlation with the amount of diosgenin in one year Some tetraploid strains showed typical polyploid traits. Conclusion Based on tissue culture, it is feasible to improve the quality of Dioscorea zingiberensis by using polyploid breeding.