采用农杆菌介导法,将Bt cry1Ia8抗虫基因转入早熟春甘蓝自交系F2011中,共获得37株卡那霉素抗性植株,其中23株经PCR检测呈阳性;Southern blot检测结果表明,cry1Ia8基因已成功整合到甘蓝基因组中;RT-PCR和Western blot检测结果表明,cry1Ia8基因在RNA水平和蛋白质水平均得到表达;对转基因植株进行ELISA检测,其Bt毒蛋白含量在201.9~241.3ng·g-1(FW)之间;离体饲虫试验结果表明,转基因植株对敏感小菜蛾和Cry1Ac抗性小菜蛾均具有较好的抗性,且Bt毒蛋白表达量越大,植株抗性越强。 Using Agrobacterium-mediated method, the Bt cry1Ia8 insect-resistant gene was transferred into early-maturing spring cabbage inbred line F2011, 37 kanamycin-resistant plants were obtained, of which 23 were positive by PCR; Southern blot results showed , cry1Ia8 gene has been successfully integrated into the genome of cabbage. The results of RT-PCR and Western blot showed that cry1Ia8 gene was expressed at both RNA and protein levels. The Bt toxin content in the transgenic plants was between 201.9 and 241.3 ng · G-1 (FW). The results showed that the transgenic plants had good resistance to both susceptible diamondback moth (Plutella xylostella) and Cry1Ac-resistant diamondback moth (Plutella xylostella) and the higher Bt toxin expression The stronger.