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目的:研究黑莓籽石油醚部位、正丁醇部位及黑莓籽油对高脂血症小鼠的脂质代谢和脂质过氧化的影响。方法:120只小鼠随机分为12组:正常组,模型组,阳性对照组和石油醚部位高剂量组(800 mg·kg-1),中剂量组(400 mg·kg-1)和低剂量组(200 mg·kg-1),正丁醇部位高剂量组(600 mg·kg-1),中剂量组(300 mg·kg-1)和低剂量组(150 mg·kg-1),黑莓籽油高剂量组(1 000 mg·kg-1),中剂量组(500 mg·kg-1)和低剂量组(250 mg·kg-1),采用灌胃高脂乳剂诱导高脂血症小鼠模型,测定血清中甘油三脂(TG),总胆固醇(TC),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C),丙二醛(MDA)的含量,超氧化物歧化酶(SOD)活性及肝、肾、脾系数。结果:石油醚部位低剂量组能显著性的降低血清中TG,TC,LDL-C和MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);正丁醇部位中、低剂量组均能显著性的降低血清中TG,TC,LDL-C,MDA的含量及肝、脾系数,升高SOD的活性(P<0.05);黑莓籽油中剂量组均能显著性的降低血清中TG,TC,MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);油高剂量组均能显著性的降低血清中TG,TC,MDA的含量及肝、脾系数,升高SOD的活性(P<0.05);正丁醇部位高剂量组能显著性的降低血清中TG,MDA的含量及肝、脾系数,升高HDL-C的含量及SOD的活性(P<0.05);油低剂量组能显著性的降低血清中TG,TC,LDL-C的含量及肝、脾系数,升高HDL-C的含量(P<0.05);石油醚部位高剂量组(TG,TC)和中剂量组(TC,HDL-C)及肝脾系数)能显著性改变,其他各指标虽不具有显著性,但与模型组相比,具有一定的效果。结论:黑莓籽石油醚部位、正丁醇部位及黑莓籽油具有一定程度的调节脂质代谢和抗脂质过氧化,改善脂质在小鼠肝脏和脾中的蓄积,减少氧化应激,推测其可能是黑莓籽有效部位降血脂的作用机制之一。
OBJECTIVE: To study the effects of blackberry seeds, n-butanol and blackberry seed oil on lipid metabolism and lipid peroxidation in hyperlipidemic mice. Methods: One hundred and twenty mice were randomly divided into 12 groups: normal group, model group, positive control group and petroleum ether high dose group (800 mg · kg -1), medium dose group (400 mg · kg -1) and low (200 mg · kg -1), n-butanol (600 mg · kg -1), middle dose (300 mg · kg -1) and low dose (150 mg · kg -1) (1 000 mg · kg -1), middle dose (500 mg · kg -1) and low dose (250 mg · kg -1) of blackberry seed oil were used to induce hyperlipidemia Serum triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) Content, superoxide dismutase (SOD) activity and liver, kidney and spleen coefficient. Results: The low dose petroleum ether group could significantly decrease the content of TG, TC, LDL-C and MDA, the index of liver and spleen, increase the content of HDL-C and the activity of SOD (P <0.05) Butanol and low dose groups could significantly reduce the contents of TG, TC, LDL-C, MDA and the index of liver and spleen, and increase the activity of SOD in serum (P <0.05) (P <0.05). The levels of TG, TC and MDA, the index of liver and spleen, the content of HDL-C and the activity of SOD in serum were significantly decreased (P <0.05) TG, TC, MDA, hepatic and spleen indexes and SOD activity (P <0.05). High-dose n-butanol group could significantly decrease the content of TG and MDA, High HDL-C content and SOD activity (P <0.05). The low-oil group could significantly decrease the content of TG, TC, LDL-C and the index of liver and spleen and increase the content of HDL- P <0.05). The levels of TG, TC and HDL-C in the petroleum ether group and the coefficient of hepatic and spleen of the petroleum ether group were significantly different, while the other indexes were not significant. However, compared with the model group Compared with a certain effect. CONCLUSION: The petroleum ether, n-butanol and blackberry seed oil of blackberry seeds can regulate lipid metabolism and lipid peroxidation to a certain extent, improve lipid accumulation in mouse liver and spleen and reduce oxidative stress. It may be one of the mechanisms of hypolipidemic effect of the effective parts of blackberry seeds.