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目的探讨羟基酪醇和橄榄苦苷在氧化应激致猪LLC-PK_1(p LLC-PK1)细胞损伤中的拮抗作用。方法用10~200μmol/L过氧化氢处理pLLC-PK_1细胞,筛选最佳的处理时间与浓度,建立LLC-PK_1细胞过氧化氢氧化应激体外模型。LLC-PK1细胞分别与2、5和10μmol的羟基酪醇和橄榄苦苷孵育12 h,用100μmol/L的过氧化氢作用4 h,裂解LLC-PK_1细胞后,检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化物酶(GSH-Px)活力和脂肪酸组成变化。结果与过氧化氢模型组(100μmol/L)相比,在橄榄苦苷和羟基酪醇处理的LLC-PK_1细胞中,MDA表达量降低,SOD和GSH-Px活力显著升高,脂肪酸转化降低,差异均有统计学意义(P<0.05,P<0.01)。结论羟基酪醇和橄榄苦苷对过氧化氢诱导的LLC-PK_1细胞氧化应激具有拮抗作用。
Objective To investigate the antagonistic effects of hydroxytyrosol and oleuropein on the oxidative stress-induced LLC-PK1 (p LLC-PK1) cell injury. Methods pLLC-PK_1 cells were treated with 10 ~ 200μmol / L hydrogen peroxide, and the optimal treatment time and concentration were screened to establish an in vitro model of oxidative stress induced by hydrogen peroxide in LLC-PK_1 cells. LLC-PK1 cells were incubated with 2, 5 and 10 μmol of hydroxytyrosol and oleuropein for 12 h, respectively. After LLC-PK 1 cells were lysed with 100 μmol / L hydrogen peroxide for 4 h, the content of malondialdehyde (MDA) Changes in superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity and fatty acid composition. Results In LLC-PK1 cells treated with oleuropein and hydroxytyrosol, the expression of MDA was decreased, the activities of SOD and GSH-Px were significantly increased and the fatty acid conversion was decreased compared with the hydrogen peroxide model group (100μmol / L) The differences were statistically significant (P <0.05, P <0.01). Conclusion Hydroxytyrosol and oleuropein have antagonistic effects on hydrogen peroxide-induced oxidative stress in LLC-PK1 cells.