Objective: To observe the clinical effect of knee-bitong prescription combined with platelet-rich plasma in the treatment of knee osteoarthritis in the middle stage. Methods: From May 2018 to January 2019, 90 patients with moderate knee osteoarthritis (K-L classification Ⅱ and Ⅲ) with liver and kidney deficiency were admitted, and 45 patients were divided into experimental group and control group. The test group included 15 males and 30 females; aged 46 to 70 (58.93 ± 10.671) years; course of disease was 1 to 8 years (27.06 ± 4.216) months; K-L classification: 33 cases in grade Ⅱ and 12 cases in grade Ⅲ. Jibitongfang Jianjian Granules is taken orally once a day, once in the morning and evening, 14 days a course of treatment, and combined with platelet-rich plasma (2ml) articular cavity injection, once every 14 days, a total of two, 14 days apart; control group male 12 There were 33 females aged 43-68 (57.00 ± 8.832) years old; the course of disease was 1-8 years (25.40 ± 3.718) months; KL classification: 36 cases of grade Ⅱ, 9 cases of grade Ⅲ, and the control group were given platelet-rich plasma ( 2ml) Injection into the joint cavity, the treatment course and frequency are the same as the experimental group. Record and compare the VAS score, WOMAC score, matrix metalloproteinase-1 and interleukin-1β levels of joint fluid before and after treatment for one month to evaluate the therapeutic effect. Results: All 90 cases were followed up without serious adverse reactions or other complications. The WOMAC scores of the experimental group and the control group before and after treatment were: 106.40 ± 47.962, 116.13 ± 41.151, 55.13 ± 22.022, 62.67 ± 26.232. The VAS scores of the two groups before and after treatment were: 5.87 ± 2.446, 5.80 ± 2.145, 2.67 ± 1.345, 4.00 ± 1.964. The levels of interleukin 1β in the test group and the control group before and after treatment were: 78.33 ± 7.227, 76.60 ± 7.069, 25.73 ± 3.239, 31.13 ± 5.743. The levels of matrix metalloproteinase-1 before and after treatment in the two groups were: 2299.66 ± 179.343, 2206.66 ± 194.235, 1741.86 ± 183.077, 1762.46 ± 192.097. Conclusion: The combination of platelet-rich plasma and knee-bitong decoction in the treatment of moderate knee osteoarthritis is better than platelet-rich plasma group in relieving knee pain, improving knee function and reducing the levels of interleukin-1β and matrix metalloproteinase-1 in joint fluid. And the side effects are small, it is worth clinical application.