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目的研究一种发病机制、病理改变与临床接近且周期较短的SD大鼠腺性膀胱炎模型制备方法与技术。方法模型组采用DH5α大肠埃希菌溶液(108~109CFU/100μL浓度),每隔2天灌注雌性SD大鼠膀胱以诱导产生腺性膀胱炎。对照组以生理盐水每隔2天灌注膀胱;空白组不采取任何干预措施,同条件饲养。45d后收集膀胱标本进行病理学检查。结果模型组膀胱标本的腺性膀胱炎发生率显著高于对照组及空白组(P<0.001);对照组与空白组其发生率差异没有统计学意义(P>0.05)。结论使用大肠杆菌多次膀胱灌注雌性SD大鼠诱导产生的腺性膀胱炎动物模型其病理表现与人类腺性膀胱炎具有相似性,适用于临床腺性膀胱炎的研究。
OBJECTIVE: To study a method and technique for the preparation of SD rat cystitis glandularis model which is characterized by pathogenesis, pathological changes and clinical closeness. Methods Model group DH5α Escherichia coli solution (108 ~ 109CFU / 100μL concentration), female SD rats were infused bladder every two days to induce cystitis glandularis. The control group was infused with normal saline every 2 days. The blank group was fed with the same conditions without any intervention. Bladder specimens were collected for pathological examination 45 days later. Results The incidence of cystitis glandularis was significantly higher in the model group than in the control group and the blank group (P <0.001). There was no significant difference between the control group and the blank group (P> 0.05). Conclusions The animal model of cystitis glandularis induced by repeated intravesical instillation of Escherichia coli in female SD rats is similar to human glandular cystitis and is suitable for the study of clinical cystitis glandularis.