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通过缺陷型腺病毒载体的介导将小鼠ＩＦＮ－γ基因转染入肿瘤细胞，经５０００ｒａｄ６０Ｃｏ照射后制备成瘤苗，观察其对实验性肺转移荷瘤小鼠的治疗效果，并对其治疗作用的免疫机理进行了研究。结果显示：经ＩＦＮ－γ基因转移瘤苗治疗后荷瘤小鼠肺部转移结节明显减少，小鼠存活期明显延长。体内免疫功能检测表明；经ＩＦＮ－γ基因转染瘤苗治疗后小鼠脾淋巴细胞诱导的ＣＴＬ，ＬＡＫ及腹腔巨噬细胞杀伤活性均明显升高，但ＮＫ活性变化不明显。本实验表明：通过腺病毒载体的介导而制备的ＩＦＮ－γ基因转染瘤苗能有效地通过激活体内抗肿瘤免疫功能而对实验性黑色素瘤肺转移有显著的治疗效果。
Mouse IFN-γ gene was transfected into tumor cells through the adenovirus-mediated deficient vectors. The tumor vaccines were prepared after irradiation with 5000rad60Co and their therapeutic effects on experimental lung metastasis-bearing mice were observed and treated. The role of the immune mechanism was studied. The results showed that the metastatic nodules in the lung of tumor-bearing mice were significantly reduced after treatment with IFN-γ gene transfer tumor vaccine, and the survival time of mice was significantly prolonged. The in vivo immune function test showed that the killing activity of CTL, LAK and peritoneal macrophages induced by spleen lymphocytes of mice treated with IFN-γ gene transfection significantly increased, but the NK activity did not change significantly. This experiment shows that transfection of IFN-γ gene vaccines prepared by adenoviral vectors can effectively treat experimental melanoma lung metastases with a significant therapeutic effect by activating antitumor immunity in vivo.