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目的:观察温阳益气活血方对冠心病大鼠模型心肌组织FGF-2及VEGF表达的影响。方法:40只SD大鼠随机分为正常组、假手术组、模型组和中药组,每组10只。采用左冠状动脉前降支中上1/3处行结扎法建立冠心病大鼠模型,造模一周后给予药物干预,共28天。治疗结束后测心功能及冠状动脉血流量,然后处死动物,取左室心肌组织,采用RT-FQ-PCR检测FGF-2及VEGF mRNA的表达水平。结果:模型组LVSP、+dp/dtmax及-dp/dtmax的绝对值及冠脉血流量较对照组和假手术组明显下降,差异具有统计学意义(P<0.05)。与模型组比较,中药组LVSP、+dp/dtmax、-dp/dtmax的绝对值及冠脉血流量增高,差异具有统计学意义(P<0.05)。模型组FGF-2和VEGF mRNA表达水平较正常组和假手术组明显升高(P<0.05),中药组FGF-2和VEGF mRNA表达水平均较模型组明显升高(P<0.05);空白组和假手术组相比,差异没有统计学意义(P>0.05)。结论:温阳益气活血方具有通过上调FGF-2和VEGF基因表达促进心肌新生血管的形成,改善心肌供血和提高心功能的作用。
Objective: To observe the effect of warming Yang and invigorating Qi and invigorating blood circulation on the expression of FGF-2 and VEGF in myocardium of coronary heart disease rats. Methods: Forty SD rats were randomly divided into normal group, sham operation group, model group and TCM group, with 10 rats in each group. A rat model of coronary heart disease was established by ligation of the upper third of the anterior descending branch of the left coronary artery. After a week of modeling, drug intervention was given for 28 days. Cardiac function and coronary blood flow were measured at the end of treatment. Animals were sacrificed and left ventricular myocardium was taken. The expression of FGF-2 and VEGF mRNA was detected by RT-FQ-PCR. Results: The absolute value of LVSP, + dp / dtmax and -dp / dtmax and the coronary blood flow in model group were significantly lower than those in control group and sham operation group (P <0.05). Compared with the model group, the absolute value of LVSP, + dp / dtmax, -dp / dtmax and coronary blood flow increased, the difference was statistically significant (P <0.05). The expression of FGF-2 and VEGF mRNA in model group was significantly higher than that in normal group and sham-operated group (P <0.05), while the expression of FGF-2 and VEGF mRNA in model group was significantly higher than that of model group (P <0.05) Compared with the sham group, there was no significant difference (P> 0.05). Conclusion: Warming Yang and Invigorating Qi and Promoting Blood Circulation Decoction has the effect of promoting the formation of myocardial neovascularization, improving myocardial blood supply and cardiac function by up-regulating FGF-2 and VEGF gene expression.