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In the present paper, the electrochemical behavior of ergosterol has been investigated by in situ circular dichroisin (CD) spectroelectrochemisty with long path-length thin layer cell. E0(1 .02V), αnα (0.302) of the electrokidation process of ergosterol were obtained from the CD spectroelectTochemical data. The mechaltisln of the electroxidation process of ergosterol is suggested.