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目的应用分子流行病学方法,以分析2名具有高危性行为的男男性接触者(MSM)间HIV-1病毒传播关系。方法对2名人类免疫缺陷病毒(HIV)感染者进行流行病学调查。应用巢式RT-PCR扩增HIV-1 pol区和gag区基因,经DNA测序后,结合流行病学资料,用遗传进化系统树比对的方法分析两感染者体内HIV-1毒株的亲缘关系。结果该2名感染者体内HIV-1毒株所属亚型分别为HIV-1 CRF07_BC和CRF01_AE,gag和pol基因之间的基因距离分别为0.125和0.096。结论该2名男男性接触者间不存在HIV-1传播关系。
Objective To investigate the relationship between HIV-1 transmission among two MSM (MSM) high-risk sexual behaviors using molecular epidemiology. Methods Two HIV-infected persons were investigated by epidemiology. Nested RT-PCR was used to amplify the HIV-1 pol region and gag region genes. After DNA sequencing and epidemiological data, the phylogenetic tree was used to analyze the relatives of HIV-1 strains relationship. Results The subtypes of HIV-1 strains were HIV-1 CRF07_BC and CRF01_AE respectively. The genetic distances between gag and pol genes were 0.125 and 0.096, respectively. Conclusion There is no HIV-1 transmission between the two MSM.