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目的:探讨蒿鳖养阴软坚方药物血清对肝星状细胞(HSC-T6)的作用。方法:MTT法分析不同剂量的蒿鳖养阴软坚方药物血清对HSC增殖的影响,并测定培养上清中Hyp含量和LDH活力,流式细胞仪检测其对HSC细胞周期的影响。结果:与正常血清组比较,蒿鳖养阴软坚方(0.82 g/kg、2.59 g/kg、8.2 g/kg)药物血清组吸光度(A)值显著降低,抑制率分别为11.26%、30.24%、44.51%;G0/G1期细胞比例增多而S期细胞比例减少;Hyp生成减少,LDH略微升高。结论:蒿鳖养阴软坚方药物血清可能通过阻抑HSC于G0/G1关卡而抑制HSC-T6增殖,同时抑制了胶原蛋白产生,且无细胞毒作用。
Objective: To investigate the effect of Artemisia turtle Yang nourishing soft Jiangan serum on hepatic stellate cells (HSC-T6). Methods: The MTT method was used to analyze the effect of different doses of Artemisia turtle Yangyin Ruanjian Recipe serum on the proliferation of HSC. Hyp content and LDH activity in the culture supernatant were determined. The effects of different concentrations on the HSC cell cycle were analyzed by flow cytometry. Results: Compared with the normal serum group, the absorbance (A) value of Artemisia annua Softgels (0.82 g / kg, 2.59 g / kg, 8.2 g / kg) serum was significantly decreased, the inhibition rates were 11.26%, 30.24% , 44.51%. The proportion of cells in G0 / G1 phase increased while the proportion of S phase cells decreased. The Hyp generation decreased and LDH increased slightly. Conclusion: Artemisia turtle Yangyin Ruanjian Recipe serum may inhibit the proliferation of HSC-T6 by inhibiting HSC at G0 / G1 checkpoint, and inhibit collagen production without cytotoxicity.