论文部分内容阅读
目的本实验观察依达拉奉对海人酸致痫大鼠海马神经元损伤的保护作用。方法选用成年健康雄性Wistar大鼠18只,体重260±20g。实验动物随机分为3组,①sham组(n=6):右侧海马CA3区注入等量的生理盐水;②KA模型组(n=6):右侧海马CA3区注入KA 4μg.kg-1(4μg/μl);③依达拉奉组(n=6):右侧海马CA3区注入KA 4μg.kg-1(4μg/μl)后,即刻给予依达拉奉10mg.kg-1.d-1腹腔注射。于大鼠注药或假手术后立即观察各组大鼠的行为学表现,于7d断头取脑,石蜡切片进行硫堇染色,于光学显微镜下观察注药对侧(左侧)海马CA1、CA3区及CA4门区组织形态学特征,并对其进行组织学分级。结果 Sham组大鼠注射对侧海马CA1、CA3和CA4门区无明显组织损伤,组织学分级多为0～1级,ND值为198±20.62和212±30.14;模型组KA致痫大鼠可见明显的组织损伤,组织学分级多为2～3级,ND值为79±13.72和90±14.98,与sham组相比,组织学分级显著升高(p<0.05),ND值显著降低(p<0.01)。依达拉奉组大鼠海马CA1区可见少量、散在性神经元坏死,组织学分级多1～2级,ND值为101±16.85和135±12.17。与模型组相比,组织学分级降低(p<0.05),ND值显著升高(p<0.05)。结论依达拉奉能够减轻KA致痫大鼠海马神经元的损伤,对神经元具有保护作用。
Objective To observe the protective effect of edaravone on hippocampal neuron injury induced by kainic acid in rats. Methods 18 adult healthy male Wistar rats weighing 260 ± 20g were selected. The experimental animals were randomly divided into three groups: ①sham group (n = 6): the right hippocampal CA3 area was injected with the same amount of saline; ②KA model group (n = 6): right hippocampal CA3 area injected KA 4μg.kg-1 (4μg / μl); ③ edaravone group (n = 6): the right hippocampal CA3 area after injection of KA 4μg.kg-1 (4μg / μl), immediately given edaravone 10mg.kg-1.d- 1 intraperitoneal injection. The rats were injected with drugs or sham operation immediately after the observation of the behavior of rats, brain and paraffin sections at 7d after decantation with thionine staining observed under light microscope contralateral (left) hippocampal CA1, The histopathological features of the CA3 and CA4 glands were histologically graded. Results There was no obvious tissue damage in CA1, CA3 and CA4 in the contralateral hippocampus of Sham rats. The histological grading were mostly grade 0 to 1, and the ND values were 198 ± 20.62 and 212 ± 30.14. Compared with sham group, the histological grade was significantly increased (p <0.05) and the ND value was significantly decreased (p <0.05). The significant difference of histological grade was grade 2 to 3, with ND values of 79 ± 13.72 and 90 ± 14.98 <0.01). In the edaravone group, a small amount of scattered neuronal necrosis was seen in the hippocampal CA1 region. The grade of histological grading was 1 ~ 2 grade. The ND values were 101 ± 16.85 and 135 ± 12.17. Compared with the model group, histological grade decreased (p <0.05), ND value increased significantly (p <0.05). Conclusion Edaravone can reduce the damage of hippocampal neurons in rats with KA-induced seizures and protect the neurons.