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Neural stem cell has a potential to differentiate into neurons, astrocytes and oligodendrocytes. It provides an invitro model to screen herbal medicines on the cellular differentiation and development level. In this work, active componentfrom gypenosides and soyasaponins was prepared to investigate their effects on the differentiation of neural stem cells.. Bothgypenosides and soyasaponins promote the differentiation of neural stem cells. This method provides speed and practicalityfor screening effective herbal medicine. It is well suited for studying the mechanism of cell differentiation and development.