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[目的]兽用抗生素被广泛用于畜禽养殖业的疾病预防与促进动物生长,由于动物对抗生素的吸收率较低,抗生素可以母体或代谢产物方式残留于畜禽粪便中,并随畜禽粪便的施用进入农田环境,对农作物生长产生潜在的影响。鉴于此,该研究以作物体内抗生素的积累与作物生长状况为观察指标评估了土壤中土霉素污染对蔬菜作物的影响。[方法]在温室内,选择了砂质土和粘质土等2种土壤,通过使用畜禽粪及添加不同剂量的土霉素(0,2,5,10,25和50 mg/kg)进行了为期7个星期的萝卜生长试验,测定了萝卜地上和地下部分中土霉素含量,观察了萝卜光合作用强度及生物量的变化,模拟研究了由畜禽粪便引入的抗生素对萝卜生长及萝卜对土壤中土霉素的吸收。[结果]萝卜能吸收土壤中的土霉素,其体内土霉素含量随土壤中土霉素污染浓度增加而增加,并随作物生长时间的增加有所下降;萝卜根系中土霉素积累比地上部分明显。砂质土壤中土霉素的生物有效性高于粘质土壤。土壤中土霉素污染水平低于10 mg/kg对萝卜生长影响不明显,但污染水平在25 mg/kg以上时可显著减弱萝卜叶片的光合作用强度,减少根系和地上部分的生物量。[结论]由于抗生素可在蔬菜中的积累,研究认为长期施用含高量抗生素的畜禽粪便可引起人类食用蔬菜的健康风险。
[Objective] Veterinary antibiotics are widely used in disease prevention and animal growth in livestock and poultry breeding industry. Due to the low absorption rate of antibiotics to animals, antibiotics can remain in animal manure in the form of maternal or metabolic products, Application of faeces enters the farmland environment, with potential impacts on crop growth. In view of this, the study of crop in vivo antibiotic accumulation and crop growth as an indicator to assess soil oxytetracycline contamination of vegetable crops. [Method] Two types of soils, sandy soil and clayey soil, were selected in greenhouse. By using animal manure and adding different doses of oxytetracycline (0, 2, 5, 10, 25 and 50 mg / kg) The radish growth experiment was conducted for 7 weeks. The contents of oxytetracycline in ground and underground parts of radish were measured. The changes of photosynthesis intensity and biomass of radish were observed. The effects of antibiotics introduced from livestock manure on radish growth and Radish on soil oxytetracycline absorption. [Result] Radish was able to absorb oxytetracycline in soil, and its oxytetracycline content increased with the concentration of oxytetracycline in soil, and decreased with the growth of crop; the accumulation ratio of oxytetracycline in radish root The ground part is obvious. The bioavailability of oxytetracycline in sandy soil is higher than that of clayey soil. Soil oxytetracycline levels less than 10 mg / kg had no significant effect on radish growth, but at 25 mg / kg, the intensity of photosynthesis of radish leaves was significantly reduced and the biomass of root and shoot decreased. [Conclusion] Due to the accumulation of antibiotics in vegetables, the study suggests that long-term application of livestock manure containing high amounts of antibiotics may cause health risks for human consumption of vegetables.