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应用喹啉酸 (每侧 1 50 nmol,2 μL,给受试药后 d3注射 )损毁老年大鼠双侧 Meynert基底核制备阿尔茨海默病 (AD)动物模型 ,放射化学法测定胆碱乙酰基转移酶 (Ch AT)活性 ,一次性训练被动回避跳台实验和水迷宫空间分辨能力测试 ,观察人参茎叶皂甙 (GSL )和胆碱合用对 AD模型大鼠学习记忆的影响 .结果显示 :GSL(40 0 mg·kg-1·d-1)和胆碱 (2 0 0 mg·kg-1· d-1)合用 ig给药 ,大鼠在跳台中出现的错误反应次数 (给药 1 3d)和学会迷宫训练次数 (给药 1 6d)显著减少 ,大脑皮层 Ch AT活性明显升高 (给药 1 9d) ,且均比两药单用效应明显 ,呈协同作用 .表明 GSL和胆碱合用有协同改善 AD模型大鼠学习记忆障碍的作用 ,其机理与协同提高Ch AT活性 ,增强中枢胆碱能神经功能有关
The animal model of Alzheimer’s disease (AD) was established by using quinolinic acid (1 50 nmol, 2 μL on each side and d3 injection on the test substance) to damage the bilateral Meynert basement nuclei in aged rats. The radiochemical method was used to determine choline acetyl Ch AT activity, passive avoidance step-down test and water maze spatial resolution test were used to observe the effects of ginseng stem and leaf saponin (GSL) and choline on learning and memory of AD model rats.The results showed that GSL The number of false reactions in rats jumping on the platform (dosing 1 day 3 d) was significantly higher than that of the control group (40 d mg · kg -1 · d -1) and choline (200 mg · kg -1 d -1) ) And maze training times (dosing for 16 days) were significantly decreased, and the activity of ChAT in the cerebral cortex was significantly increased (administration for 19 days), both of which showed a synergistic effect compared with that of the two drugs alone, indicating that synergistic effect of GSL and choline There is a synergistic effect in improving learning and memory dysfunction in AD model rats, and its mechanism is related to synergistic enhancement of ChAT activity and enhancement of central cholinergic function