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为了将籼稻品种 Ｃｈｉｎｓｕｒａｈ Ｂｏｒｏ Ⅱ（ＣＢⅡ）的细胞质雄性不育特性转移到粳稻品种Ｎｉｐｐｏｎｂａｒｅ中，用电融合的方法诱导碘乙酰胺失活的Ｎｉｐｐｏｎｂａｒｅ原生质体与经Ｘ－射线处理的ＣＢＩＩ原生质体的融合。在具有二倍体染色体数目的１６个融合衍生植株中，其中一个完全雄性不育，形态学上与Ｎｉｐｐｏｎｂａｒｅ一致，其雄性不育性能稳定地传递给全部回交后代，这为雄性不育的母性遗传提供了证据，而且回交后代的细胞质雄性不育性可被来源于ＣＢⅡ的育性恢复基因Ｒｆ１以配子体遗传的方式所恢复。限制性片段和Ｓｏｕｔｈｅｒｎ ｌｏｔ分析揭示了雄性不育植株中存在来源于ＣＢⅡ的线粒体质粒ＤＮＡ。我们的研究表明用细胞融合把ＣＭＳ转移到所需要的水稻品种中能用于未来杂交种子的生产。
In order to transfer the cytoplasmic male sterility of indica variety Chinsurah Boro Ⅱ (CB Ⅱ) into japonica variety Nipponbare, the fusion of iodoacetamide inactivated Nipponbare protoplasts and X-ray-treated CBII protoplasts was induced by electrofusion . Of the 16 fusion-derived plants with diploid chromosome numbers, one of them was completely male sterile, morphologically consistent with Nipponbare, and its male sterility was consistently transmitted to all backcross progeny, which is male sterile Heredity provided evidence that the cytoplasmic male sterility of backcross progeny was restored in a gametophytic manner by the fertility restorer gene Rf1 from CBII. Restriction fragments and Southern blot analysis revealed the presence of CBII-derived mitochondrial plasmid DNA in male-sterile plants. Our research shows that the use of cell fusion to transfer CMS to the desired rice variety can be used for future hybrid seed production.