采用双波长分光光度法测定复方替硝唑褥疮膜中替硝唑与盐酸小檗碱的含量 ,替硝唑的测定波长为 32 0nm ,参比波长为 357nm ;盐酸小檗碱的测定波长为 32 8 5nm ,参比波长为 30 5 5nm。结果平均回收率分别为 97 81% (RSD =1.88% ,n =5)和 98 87% (RSD =0 .31% ,n =5)。处方中的其它成分对测定未见干扰。 The determination of tinidazole and berberine hydrochloride in compound tinidazole decubitus membrane by dual-wavelength spectrophotometry was carried out. The determination wavelength of tinidazole was 320 nm and the reference wavelength was 357 nm. The determination wavelength of berberine hydrochloride was 32 8 5nm, the reference wavelength of 30 5 5nm. Results The average recoveries were 97 81% (RSD = 1.88%, n = 5) and 98% (RSD = 0.31%, n = 5), respectively. Other ingredients in the prescription did not interfere with the assay.