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目的:探讨C/EBPα在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其与肿瘤微血管密度(microvesseldensity,MVD)的关系。方法:应用免疫组织化学EnVision法检测40例手术切除的NSCLC组织及其配对的40例距肿瘤>5 cm以上癌旁正常肺组织中C/EBPα蛋白的表达,并分析C/EBPα在NSCLC中的表达与其临床病理特征的关系。采用CD34标记肺癌组织中的肿瘤微血管,分析C/EBPα的表达与肿瘤MVD的关系。结果:NSCLC组织中C/EBPα的阳性表达率明显低于癌旁正常肺组织(P<0.05)。C/EBPα与NSCLC患者的年龄、性别、TNM分期及有无淋巴结转移均无关(P>0.05),与NSCLC组织的分化程度及病理类型有关(P<0.05)。在NSCLC组织中,C/EBPα蛋白表达阳性组MVD明显低于C/EBPα蛋白表达阴性组(P<0.05)。结论:C/EBPα在NSCLC中的低表达可能通过调节MVD介导NSCLC的发生和发展。
Objective: To investigate the expression of C / EBPα in non-small cell lung cancer (NSCLC) and its relationship with microvessel density (MVD). Methods: EnVision immunohistochemical method was used to detect the expression of C / EBPα protein in 40 surgically resected NSCLC tissues and matched 40 normal lung tissues> 5 cm above the tumor. The expression of C / EBPα in NSCLC Expression and its clinicopathological features. The tumor microvessels in lung cancer tissues were labeled with CD34, and the relationship between C / EBPα expression and tumor MVD was analyzed. Results: The positive rate of C / EBPα in NSCLC tissues was significantly lower than that in normal lung tissues (P <0.05). C / EBPα was not associated with age, sex, TNM stage and lymph node metastasis (P> 0.05) in patients with NSCLC, but also with the degree of differentiation and pathological type of NSCLC (P <0.05). In NSCLC, MVD of C / EBPα-positive group was significantly lower than that of C / EBPα-negative group (P <0.05). Conclusion: The low expression of C / EBPα in NSCLC may mediate the occurrence and development of NSCLC by regulating MVD.