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目的观察大蒜素对直肠癌细胞凋亡影响。方法直肠癌的患者随机分为对照组、大蒜素组,并选取正常人作为正常组共3组。入院后大蒜素组予大蒜素60mg/d静脉滴注,1次/d,连用7d,直至手术,无其他化疗措施。留取肠组织标本以4%多聚甲醛固定,石蜡包埋,4μm连续切片,HE染色,光镜下观察,并行TUNEL染色了解各组的细胞凋亡情况。结果与对照组相比,大蒜素组中的细胞凋亡数量明显增加(P<0·05)。结论大蒜素可通过增加细胞凋亡而抑制肿瘤细胞的发生、发展。
Objective To observe the effect of allicin on apoptosis of rectal cancer cells. Methods Patients with rectal cancer were randomly divided into control group, allicin group, and normal subjects were selected as normal group. Allicin after admission to allicin 60mg / d intravenous infusion, 1 / d, once every 7d, until surgery, no other chemotherapy. The specimens of intestinal tissue were collected and fixed with 4% paraformaldehyde, embedded in paraffin, sliced at 4μm, stained with hematoxylin-eosin (HE) and observed under light microscope. TUNEL staining was used to observe the apoptosis in each group. Results Compared with the control group, the number of apoptotic cells in allicin group increased significantly (P <0.05). Conclusion Allicin can inhibit the occurrence and development of tumor cells by increasing apoptosis.