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Two new compounds involving a thiophene moiety named as 2,5-bis[4-(N,N- diphenylamino)styryl]thiophene (BPST) and 2,5-bis[4-(N,N-diethylamino)styryl]thiophene (BEST) have been synthesized. The two-photon absorption cross section of BPST was measured as large as 256×10-50 cm4·s/photon, when it was excited by 800 nm femtosecond laser.