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用高效液相色谱 (HPLC)测定方法研究洛美沙星在鲤鱼 (Cyprinuscarpio)体内的药物动力学和残留。洛美沙星以 2 0mg/kg体重单剂量口灌鲤鱼后 ,测定其血浆药物浓度 ,最低检测限为 0 0 5 μg/mL ,动力学模型符合开放性有吸收二室模型 ,药物动力学参数 :表观分布容积 (Vd/F)、吸收半衰期 (tl/ 2kα)、分布半衰期 (t1/ 2α)以及消除半衰期 (t1/ 2 β)分别为 5 2 1L/kg、 0 31h、 1 0 2h、 2 0 0 3h ,其药时曲线下面积(AUC)为 110 92 μg·h/mL。以相同剂量单次口灌鲤鱼后 ,在不同的天数测定了鲤鱼皮肤、肌肉和肝胰脏中洛美沙星的残留 ,在给药 12天后 ,洛美沙星在各组织中均能检测到且残留均低于 0 0 8μg/g ,结果表明洛美沙星在鲤鱼体内消除缓慢、残留期较长 ,休药期不低于 12天。
The pharmacokinetics and residues of lomefloxacin in Cyprinus carpio were studied by high performance liquid chromatography (HPLC). Lomefloxacin 20 mg / kg body weight single oral dose of carp, measured plasma drug concentration, the minimum detection limit of 0 0 5 μg / mL, kinetic model in line with the open absorption of two compartment model, pharmacokinetic parameters: The apparent distribution volume (Vd / F), absorption half-life (t1 / 2kα), distribution half-life (t1 / 2α) and elimination half life (t1 / 2β) were 5 2 1L / kg, 0 31h, 1 0 2h, 0 0 3h, the area under the curve (AUC) was 110 92 μg · h / mL. After a single oral dose of common carp at the same dose, the residues of lomefloxacin in carp skin, muscle, and hepatopancreas were determined at different days, and lomefloxacin was detected in all tissues and remained after 12 days Were lower than 0 0 8μg / g, the results showed that lomefloxacin in the carp to eliminate slowly, longer residual period, withdrawal period of not less than 12 days.