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[目的]探讨食管鳞癌(ESCC)患者癌组织的基因表达谱,进一步寻找在ESCC及切缘组织中差异表达的基因。[方法]取3例无ESCC家族史患者癌及切缘组织等量混合,抽提RNA,将RNA逆转录合成相应cDNA,以Cy5和Cy3标记cDNA作为探针,在含有14000点人类基因组芯片(BiostarH-140s)上进行杂交。用scanarray4000扫描仪扫描芯片荧光信号图象,用GenePix Pro3.0软件对扫描图象进行数字化处理和分析。[结果]依Ratio(cy5/cy3)>2.0或<0.5的数据项为差异表达的基因分别为1855个。含表达序列标签(EST)844个,下调基因378个,上调基因466个。在1011个已知功能的基因中,下调基因560个,上调基因451个。包含各类功能基因。同时与6例具家族史ESCC组织差异基因进行了初步分析,结果初步表明有和无家族史患者基因表达谱不完全相同。经与NCBI基因库比对,有8个基因相同,且异常表达与报道相符。[结论]基因芯片是一高效筛选食管癌相关基因的方法。在ESCC的发生、发展中,存在着大量异常表达基因。
[Objective] To investigate the gene expression profiles in esophageal squamous cell carcinoma (ESCC) patients and to find out the differentially expressed genes in ESCC and marginal tissues. [Method] Three cancer patients with no family history of ESCC and their marginal tissues were mixed in the same volume. RNA was extracted and cDNA was reverse transcribed into cDNA. Cy5 and Cy3-labeled cDNAs were used as probes. The cDNAs containing 14000 human genomic microarrays Biostar H-140s). Scanarray4000 scanner used to scan the chip fluorescent signal images, using GenePix Pro3.0 software to digitize the scanned images and analysis. [Results] The number of differentially expressed genes according to Ratio (cy5 / cy3)> 2.0 or <0.5 was 1855, respectively. 844 ESTs, 378 down-regulated genes and 466 up-regulated genes. Of the 1011 known genes, 560 were down-regulated and 451 were up-regulated. Contains a variety of functional genes. At the same time with 6 cases of family history of ESCC tissue differential genes were initially analyzed, the results initially showed that patients with and without family history gene expression profiles are not exactly the same. After comparing with NCBI gene library, 8 genes were identical, and the abnormal expression was consistent with the report. [Conclusion] Gene chip is an efficient method for screening esophageal cancer related genes. In the occurrence and development of ESCC, there are a large number of abnormally expressed genes.