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目的探讨非选择性环氧化酶抑制剂氟比洛芬酯对大鼠局灶性脑缺血再灌注损伤后血浆血栓素A_2(TXA_2)和前列环素I_2(PGI_2)水平的影响。方法采用线栓法建立大鼠大脑中动脉闭塞2 h/再灌注24 h动物模型。24只♂SD大鼠随机分成4组,每组6只:假手术组、生理盐水对照组、氟比洛芬酯6和12 mg·kg~(-1)组。于插入线栓前10 min分别给予2mL生理盐水、氟比洛芬酯6和12 mg·kg~(-1)。大脑中动脉闭塞2h/再灌注24 h后采集大鼠血浆标本,放射免疫法检测血浆TXA_2的代谢产物血栓素B_2(TXB_2)和PGI_2的代谢产物6-酮-前列腺素_(1α)(6-keto-PGF_(1α))浓度。结果大脑中动脉闭塞2h/再灌注24 h后对照组与假手术组相比,TXB_2浓度显著增加[(1 038.12±110.02)ng·L~(-1) vs(341.29±47.98)ng·L~(-1),P<0.01],6-keto-PGF_(1α)显著减少[(539.79±45.07)ng·L~(-1) vs(1 268.24±131.01)ng·L~(-1),P<0.01]。氟比洛芬酯6及12 mg·kg~(-1)组与对照组相比能显著降低血浆TXB_2浓度[(605.53±45.27)、(495.46±66.03)ng·L~(-1),P<0.01],增加血浆6-keto-PGF_(1α)浓度[(839.34±60.22)、(963.08±82.86)ng·L~(-1),P<0.01]。氟比洛芬酯12 mg·kg~(-1)比6 mg·kg~(-1)效果更为显著。结论氟比洛芬酯能减轻大鼠脑缺血再灌注损伤后血浆TXA_2和PGI_2的变化。
Objective To investigate the effects of flurbiprofen axetil, a nonselective cyclooxygenase inhibitor, on the levels of TXA_2 and PGI_2 in rats with focal cerebral ischemia-reperfusion injury. Methods The middle cerebral artery occlusion (2 h) / reperfusion (24 h) animal models were established by thread occlusion. Twenty-four male SD rats were randomly divided into 4 groups with 6 rats in each group: sham operation group, normal saline control group, flurbiprofen axetil 6 and 12 mg · kg -1 groups. Two mL of normal saline, flurbiprofen axetil 6 and 12 mg · kg ~ (-1) were given 10 minutes before the thread plug was inserted. Plasma samples were collected from the middle cerebral artery occlusion for 2h and reperfusion for 24 h, and the metabolites of thromboxane B_2 (TXB_2) and PGI_2 (6-keto-PGF1α) keto-PGF_ (1α)) concentration. Results Compared with the sham-operation group, TXB_2 concentration was significantly increased in the middle cerebral artery occlusion 2h / reperfusion 24 h ([(1038.12 ± 110.02) ng · L -1 vs (341.29 ± 47.98) ng · L -1) (539.79 ± 45.07) ng · L -1 vs (1 268.24 ± 131.01) ng · L -1, P <0.01), P <0.01] P <0.01]. Flurbiprofen Ester 6 and 12 mg · kg -1 could significantly decrease the TXB_2 concentration in plasma ([(605.53 ± 45.27), (495.46 ± 66.03) ng · L -1, P (P <0.01). The plasma concentration of 6-keto-PGF_ (1α) [(839.34 ± 60.22), (963.08 ± 82.86) ng · L -1, P 0.01]. Flurbiprofen axetil 12 mg · kg -1 was more effective than 6 mg · kg -1. Conclusion Flurbiprofen axetil can reduce the changes of plasma TXA_2 and PGI_2 after cerebral ischemia-reperfusion injury in rats.