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目的研究巨噬细胞迁移抑制因子(macrophage migration inhibitory factor,MIF)基因单核苷酸多态性(SNPs;rs755662,rs11548059,rs1049829,rs1803976)与结直肠癌发生风险的关系。方法收集共计192例结直肠癌患者(CRC)和256名正常对照者外周血样本,以聚合酶链反应和Taqman探针分析方法,检测MIF基因单核苷酸多态性;以Logistic回归模型计算不同基因型与结直肠癌发生风险的关系。结果 rs755662基因型的出现频率在CRC组和正常对照组之间差异有统计学意义(P=0.011),而在rs11548059、rs1049829和rs1803976位点则差异无统计学意义(P=0.660、P=0.700和P=0.959)。此外,rs755662还分别与早期发病(年龄≤50岁,P=0.026)、分期(Ⅳ期,P=0.038)以及分化(P=0.040)有关。与正常对照组比,rs755662与Ⅲ期和Ⅳ期显著相关(P值分别为0.034和0.003)。结论 MIF基因5′-UTR区域rs755662(G/C)单核苷酸多态性与结直肠癌的易感性、患者发病年龄和分期有关。
Objective To investigate the association between the single nucleotide polymorphism (MPS) of macrophage migration inhibitory factor (MIF) and the risk of colorectal cancer (rs755662, rs11548059, rs1049829, rs1803976). Methods Peripheral blood samples were collected from 192 patients with CRC and 256 healthy controls. Polymerase chain reaction and Taqman probe were used to detect single nucleotide polymorphisms of MIF gene. Logistic regression model was used to calculate Relationship between Different Genotypes and Risk of Colorectal Cancer. Results The frequency of rs755662 genotype was significantly different between CRC group and normal control group (P = 0.011), while there was no significant difference between rs11548059, rs1049829 and rs1803976 (P = 0.660, P = 0.700 And P = 0.959). In addition, rs755662 was also associated with early onset (age ≤50 years, P = 0.026), stage (stage Ⅳ, P = 0.038), and differentiation (P = 0.040). Compared with the normal control group, rs755662 was significantly associated with stage III and IV (P = 0.034 and 0.003, respectively). Conclusion The single nucleotide polymorphism of rs755662 (G / C) in the 5’-UTR region of MIF gene is associated with the susceptibility to colorectal cancer and the age and stage of onset of the disease.