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目的:探讨荷叶水提物对氧化低密度脂蛋白(Ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)表达单核细胞趋化蛋白1(MCP-1)和血管细胞黏附分子1(VCAM-1)的影响。方法:(1)分别用25mg/L、50mg/L、100mg/L的Ox-LDL刺激培养的HUVECs 24h,半定量逆转录聚合酶链反应(RT-PCR)方法测定MCP-1和VCAM-1的mRNA表达,酶联免疫吸附法(ELISA)法测定细胞上清中MCP-1和VCAM-1的含量;(2)预先分别用10mg/L、20mg/L、40mg/L的荷叶水提物干预HUVECs,再用50mg/L的Ox-LDL刺激,半定量RT-PCR方法测定MCP-1和VCAM-1的mRNA表达,ELISA法测定细胞上清中MCP-1和VCAM-1的含量。结果:不同浓度的Ox-LDL刺激HUVECs后,MCP-1和VCAM-1 mRNA的表达及细胞上清中的含量呈浓度依赖性上调(P<0.01),且不同浓度的Ox-LDL组间差异亦有统计学意义(P<0.01)。荷叶水提物干预后,HUVECs的MCP-1 mRNA和VCAM-1 mRNA的表达及细胞上清MCP-1和VCAM-1含量呈浓度依赖性下降。结论:荷叶水提物能够抑制Ox-LDL诱导的HUVECs表达MCP-1和VCAM-1,可能通过这一机制有抗动脉粥样硬化的潜在作用。
OBJECTIVE: To investigate the effects of lotus leaf water extract on the expression of monocyte chemoattractant protein 1 (MCP-1) and vascular cell adhesion molecule 1 (VCAM) in human umbilical vein endothelial cells (HUVECs) induced by Oxidized Low Density Lipoprotein -1). Methods: (1) The cultured HUVECs were stimulated with 25 mg / L, 50 mg / L and 100 mg / L Ox-LDL respectively for 24 h and the expressions of MCP-1 and VCAM-1 were detected by semi-quantitative reverse transcription polymerase chain reaction The mRNA expression of MCP-1 and VCAM-1 in the cell supernatant was determined by enzyme-linked immunosorbent assay (ELISA). (2) Water extracts of lotus leaf with 10mg / L, 20mg / L and 40mg / HUVECs were treated with 50 mg / L Ox-LDL. The mRNA expressions of MCP-1 and VCAM-1 were determined by semi-quantitative RT-PCR and the levels of MCP-1 and VCAM-1 were measured by ELISA. Results: The expression of MCP-1 and VCAM-1 mRNA and the content of cell supernatant in HUVECs stimulated by different concentrations of ox-LDL were increased in a concentration-dependent manner (P <0.01), and the difference of Ox-LDL between different concentrations Also statistically significant (P <0.01). After the intervention of lotus leaf water extract, the expression of MCP-1 mRNA and VCAM-1 mRNA and the contents of MCP-1 and VCAM-1 in HUVECs were decreased in a concentration-dependent manner. Conclusion: The aqueous extract of lotus leaf can inhibit the expression of MCP-1 and VCAM-1 induced by Ox-LDL in HUVECs, which may have the potential of anti-atherosclerosis through this mechanism.