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目的采用HPLC法测定益肤丸中的落新妇苷。方法采用Inertsil ODS-3色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%冰乙酸(39∶61),流速1.0 m L·min-1,柱温30℃,进样量20μL,检测波长291 nm。结果落新妇苷10.04~200.88μg·m L~(-1)与峰面积的线性关系良好(r=0.9999),平均加样回收率为100.44%,RSD=2.73%。结论所用方法简单、快速、可靠,可用于益肤丸的质量控制。
Objective To determine the content of astilbin in Yifeu Wan by HPLC. Methods Inertsil ODS-3 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-0.1% glacial acetic acid (39:61) at a flow rate of 1.0 mL · min- 20μL, detection wavelength 291 nm. Results The linear range of 10.0 ~ 200.88μg · m L -1 of astilbin was good (r = 0.9999). The average recovery was 100.44% and RSD was 2.73%. Conclusion The method used is simple, rapid and reliable and can be used for the quality control of Yiku Wan.