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[目的 ]探讨雌激素受体拮抗剂在非小细胞肺癌治疗中的作用。 [方法 ]用逆转录多聚酶链式反应 (RT-PCR)测定肺腺癌细胞株 SPC- A- 1的雌激素受体 m RNA(ER- m RNA)表达 ,分别在去激素培养条件下观察不同浓度的他莫昔芬 (Tam)、4-羟基他莫昔芬 (OHTam)和雌二醇 (E2 )对其生长速度、细胞周期时相、ER- m RNA水平和细胞形态学的影响。 [结果 ] SPC- A- 1肺腺癌细胞株有 ER- m RNA表达 ,低浓度 E2 (1 0 -8mol/ L)可促进其生长 ,Tam(1 0 -6mol/ L )和 OHTam(1 0 -6mol/ L)可抑制 E2 (1 0 -8mol/ L )的生长刺激作用。去激素环境下细胞生长明显减慢 ,在无激素环境中亦能抑制细胞生长 ,抑制发生在 G0 / G1 期 ,并有细胞内质网扩张等结构改变 ,ER- m RNA表达水平下降。 [结论 ]肺腺癌 SPC- A- 1细胞株的生长具有雌激素依赖性 ,应用 ER拮抗剂 Tam和 OHTam能抑制其生长。肺癌细胞中 ER水平存在自身调节现象
[Objective] To explore the role of estrogen receptor antagonists in the treatment of non-small cell lung cancer. [Method] The expression of estrogen receptor m RNA (ER-m RNA) in lung adenocarcinoma cell line SPC-A-1 was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) Concentration of Tamoxifen (Tam), 4-hydroxytamoxifen (OHTam) and estradiol (E2) on its growth rate, cell cycle phase, ER-m RNA level and cell morphology. [Results] The expression of ER-m RNA in SPC-A-1 lung adenocarcinoma cell line was significantly inhibited. E2 (10 -8 mol / L) -6mol / L) can inhibit E2 (10 ~ 8mol / L) growth stimulating effect. In the absence of hormone, the growth of cells was significantly slowed down in hormone-deprived environment, the cell growth was also inhibited in G0 / G1 phase and the structure of cell endoplasmic reticulum was dilated, and the expression of ER-m RNA was decreased. [Conclusion] The growth of SPC-A-1 cells in lung adenocarcinoma is estrogen-dependent. The use of Tam antagonist Tam and OHTam can inhibit its growth. ER levels in lung cancer cells have their own regulation