论文部分内容阅读
通过根癌农杆菌(Agrobacterium tumefaciens)介导法,以番茄叶片作外植体,将番茄(Sola-num lycopersicum L.)反义光敏色素A(PHYA)基因片段导入番茄。通过PCR扩增、Sourthernblot检测,证明反义光敏色素A的基因片段已整合到番茄基因组。在转基因番茄材料中,PHYA基因表达受到抑制,番茄红素的合成显著减少,果实没有表现出正常的红色果皮;转基因果实成熟过程中乙烯能够正常合成,与对照番茄之间没有显著差异。推测在调控番茄红素合成的模式中,光敏色素可能位于乙烯的下游位点起作用,二者共同调控番茄红素的合成。
The tomato (Sola-num lycopersicum L.) antisense phytochrome A (PHYA) gene fragment was introduced into tomato by Agrobacterium tumefaciens mediated method. By PCR amplification, Sourthernblot test proved that the antisense phytochrome A gene fragment has been integrated into the tomato genome. In the transgenic tomato material, the expression of PHYA gene was inhibited, the synthesis of lycopene was significantly reduced, and the fruit did not show the normal red peel; ethylene could be synthesized normally during the maturation of transgenic fruits, and there was no significant difference with the control tomato. It is speculated that in the regulation of lycopene synthesis mode, the phytochromes may be located in the ethylene downstream sites play a role, the two together regulate the synthesis of lycopene.