论文部分内容阅读
个体血小板表面CD36抗原缺乏在随机输注时有产生抗-CD36免疫反应的风险,是血小板输注无效的原因之一。本研究应用流式细胞术检测杭州地区单采血小板供者的血小板表面CD36抗原表达情况,并分析个体血小板上CD36缺失表型的频率。留取献血者新鲜抗凝血样,经离心获取富血小板血浆,洗涤并调整血小板计数至1×106。采用CD36-FITC、CD41-PE单克隆抗体和血小板孵育反应,然后用流式细胞仪检测和分析血小板表面糖蛋白CD36抗原表达情况。对于血小板表面CD36抗原阴性的标本,进一步筛查其单核细胞表面CD36的表达情况。结果表明:192例无偿献血者筛查出7例血小板表面CD36抗原阴性,CD36缺失型频率为3.6%,均为Ⅱ型缺失。人群中个体CD36抗原表达强度存在差异,参照CD36几何平均荧光强度数值大小,59例为低表达,126例为高表达。结论:人群中存在CD36Ⅱ型缺失表型,这些数据将为研究CD36抗原分布提供参考,有助于解决血小板输注无效问题。
The lack of CD36 antigen on individual platelets is associated with the risk of developing an anti-CD36 immune response during randomized infusion and is one of the causes of ineffective platelet transfusions. In this study, flow cytometry was used to detect platelet surface CD36 antigen expression in apheresis patients in Hangzhou and to analyze the frequency of CD36 null phenotypes on individual platelets. Blood samples were collected from fresh anticoagulated blood donors, centrifuged to obtain platelet-rich plasma, washed and adjusted to a platelet count of 1 × 106. CD36-FITC, CD41-PE monoclonal antibody and platelet incubation reaction, and then detected by flow cytometry and platelet surface glycoprotein CD36 antigen expression. CD36 antigen-negative platelet surface specimens, and further screening of monocyte CD36 expression. The results showed that CD36 antigen was negative on the platelet surface of 7 out of 192 unpaid blood donors. The frequency of CD36 deletion was 3.6%, both of which were type Ⅱ deletion. There is a difference in the expression level of CD36 antigen between individuals. According to the geometric mean fluorescence intensity of CD36, 59 cases showed low expression and 126 cases showed high expression. Conclusion: There is a CD36 Ⅱ deletion phenotype in the human population. These data will provide a reference for studying the distribution of CD36 antigen and help to solve the problem of invalid platelet transfusion.