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通过长期定位试验（1986～1997年），采用正交旋转回归设计，在黑土地区不同培肥方式条件下，对土壤氮素持续效应进行研究。研究表明：培肥阶段除不施肥区外,土壤全氮量都有所增加。进入耗竭阶段后,土壤全氮又不同程度的下降,只是不同处理变化速度不同而已。不同培肥方式的全N变化，在培肥阶段的贡献和耗竭阶段的残效，均依次为农肥(化肥(秸杆。
Through long-term experiment (from 1986 to 1997), orthogonal rotation regression design was used to study the persistent effect of soil nitrogen under different fertilizing modes in the black soil region. The research shows that except for the non-fertilization area, the total nitrogen content in the fertilization stage has increased. Into the depletion phase, the soil total nitrogen decreased to varying degrees, but different processes vary in speed only. All N changes in the way of fertilizing, the contributions in the fertilizing stage and the residual effects in the depleting stage, followed by agricultural fertilizer (fertilizer (straw.