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目的 探讨 p5 3基因突变与喉癌生物学行为的关系。方法 应用聚合酶链反应 -单链构象多态性 (PCR- single strand conformation polymorphism,PCR- SSCP)银染技术结合 DNA直接测序 ,检测喉鳞癌新鲜或冰冻组织中 p5 3外显子 5～ 8的基因突变。结果 - 、 - 喉鳞癌 p5 3基因突变率分别为6 9. 2 % (18/2 6 )和 85 .3% (2 9/34 ) ,P>0 .0 5 ;高、中、低分化程度的突变率依次为 5 2 .9% (9/17)、83.3%(2 0 /2 4)和 94.7% (18/19) ,P<0 .0 5 ;有、无颈淋巴结转移者突变率分别为 96 .4% (2 7/2 8)和 6 2 .5 % (2 0 /32 ) ,P<0 .0 1。从 47例 SSCP阳性标本中随机抽取 2 0例进行测序 ,19例测序为阳性 ,二者符合率为 95 %。结论 p5 3基因突变与病理分化程度及颈淋巴结转移有明显相关性。
Objective To investigate the relationship between the mutation of p5 3 gene and the biological behavior of laryngeal cancer. Methods PCR-SSCP and silver direct sequencing were used to detect the expression of p5 exon 5 ~ 8 in fresh or frozen laryngeal squamous cell carcinoma Gene mutation. Results - The mutation rates of p5 3 gene in laryngeal squamous cell carcinoma were 69.2% (18/2 6) and 85.3% (29/34), respectively, P> 0.05. The high, moderate and poorly differentiated The rate of mutation was 52.9% (9/17), 83.3% (20/24) and 94.7% (18/19), respectively, P <0.05. There was no mutation in patients with cervical lymph node metastasis Rates were 96.4% (2 7/2 8) and 62.5% (20/32), respectively, P <0. Twenty cases were randomly selected from 47 SSCP positive samples for sequencing, and 19 cases were positive for sequencing. The coincidence rate was 95%. Conclusion The mutation of p5 3 has a significant correlation with the degree of pathological differentiation and cervical lymph node metastasis.