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目的 探讨高原人体体力负荷前、后能量代谢的变化以及高压氧预处理(hyperbaric oxygen preconditioning,HBOP)的干预作用.方法 选取由平原入伍进驻到海拔3800m高原地区6 ～24个月的健康男性战士6名,分别在HBOP前、预处理5d和7d后测定其体力负荷前、后的血糖(glucose,Glu)、乳酸(blood lactate,BLA)、乳酸脱氢酶(lactate dehydrogenase,LDH)和Na+K+-ATP酶(Na+-K+-ATPase)水平.受试者体力负荷的方式为:踏阶运动5 min,25次/min,阶高30 cm,用节拍器控制踏阶节奏和计时,劳动强度相当于650 kg · m/min.结果 经HBOP后,人体安静时的Glu、BLA、LDH和Na+-K+-ATPase较预处理前未见显著改变(P＞0.05);而HBOP 5 d、7d后负荷状态的Glu[(5.31±0.27)、(4.61±0.26) mmol/L]较负荷状态预处理前[(4.23±0.25) mmol/L]明显升高,差异有统计学意义(P ＜0.05或P＜0.01),BLA,LDH和Na+-K+-ATPase HBOP 5 d负荷后分别为(1.00±0.03) mmol/L、(216.33±16.87) U/L、(5.65±0.51) μmolPi/mgpr.h,HBOP 7 d负荷后分别为(0.92 ±0.04) mmol/L、(192.38±15.06)U、(5.38±0.38) μmolPi/mgpr.h],均较同状态预处理前[(1.08±0.07) mmol/L、(244.24 ±21.82)U、(6.49±0.46) μmolPi/mgpr.h]显著降低,差异均有统计学意义(P＜0.05或P＜0.01);HBOP 5 d组各指标较HBOP7 d组未见显著改变(P＞0.05).结论 短期HBOP即可明显改善高原战士体力负荷时的能量代谢,加速糖原分解增加Glu含量,同时减少体力负荷后的BLA堆积,降低LDH和Na+-K+-ATPase活力,使肌肉具有更高的氧利用效率,从而达到减轻疲劳和提高劳动效率的目的.“,”Objective To investigate changes in human energy metabolism both before and after physical load under high altitude hypoxia and effects of hyperbaric oxygen preconditioning (HBOP).Methods Six healthy male soldiers who used to live in the plain region,joined the army,and moved to the plateau region 6 to 12 months ago were enrolled for our study.The levels of glucose (Glu),blood lactate (BLA),lactate dehydrogenase(LDH) and Na+-K+-ATPase both before and after physical load were detected before HBOP,and 5 and 7 days of HBOP as well.The mode of physical exercise was 5-minute stair-climbing,at a rate of 25 stairs a minute,with the height of the stair being 30 cm.A pacemaker was used to control the rate of climbing up the stairs and record the time,with the physical intensity being equivalent to 650 kg · m/min.Results Following HBOP,no signficant changes could be found in the levels of Glu,BLA,LDH and Na +-K+-ATPase at rest,when compared with those before HBOP (P ＞ 0.05).However,5 and 7 days after physical load,the levels of Glu [(5.31 ±0.27) and(4.61 ±0.26) mmol/L] were significantly increased,as compared with those before HBOP [(4.23 ± 0.25) mmol/L],with statistical signficance (P ＜ 0.05 or P ＜ 0.01).The levels of BLA,LDH and Na+-K+-ATPase after 5-day HBOP were [(1.00 ± 0.03) mmol/L,(216.33 ± 16.87)U and(5.65 ±0.51) μmolPi/mgpr.h],and the levels of BLA,LDH and Na+-K+-ATPase after 7-day HBOP were [(0.92 ± 0.04) mmol/L,(192.38 ± 15.06) U and (5.38 ± 0.38) μmolPi/mgpr.h],which were obviously lower than those before HBOP [(1.08 ± 0.07) mmol/L,(244.24 ± 21.82) U and (6.49± 0.46) μmolPi/mgpr.h],also with statistical signficance (P ＜ 0.05 or P ＜ 0.01) Conclusions Short-term HBOP could significantly improve energy metabolism of military personnel at high altitude with physical load,accelerate glycogenolysis and elevate Glu level,lessen the accumulation of BLA after physical load,decrease the activity of LDH and Na+-K+-ATPase and enable muscles to make better use of oxygen,thus reducing fatigue and improving working efficiency.