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目的探讨支气管哮喘患者诱导痰中白细胞介素-9(IL-9)的水平及其与炎症细胞和肺功能的关系。方法分别选择28例支气管哮喘急性发作患者(哮喘急性发作组)和28例健康对照(健康对照组),分别检测哮喘急性发作期和治疗后缓解期及健康对照组的肺功能;并用酶联免疫吸附实验(ELISA)测定诱导痰上清液中IL-9的水平。结果哮喘发作组患者诱导痰中IL-9水平明显高于哮喘缓解期,比较差异有统计学意义(P<0.05),哮喘发作组和缓解期组痰中IL-9水平高于健康对照组,比较差异有统计学意义(P<0.05)。哮喘发作组患者诱导痰中IL-9水平与中性粒细胞数及嗜酸性粒细胞数均呈正相关(r值分别为0.68、0.81,P<0.05),与第一秒用力呼气容积占预计值的百分比(FEV%)呈负相关(r=-0.65,P<0.05)。结论支气管哮喘患者诱导痰中增高的IL-9水平与其气道炎症和气流受限有关。
Objective To investigate the level of interleukin-9 (IL-9) in sputum induced by bronchial asthma and its relationship with inflammatory cells and lung function. Methods Twenty-eight patients with acute exacerbation of bronchial asthma (acute exacerbation group) and 28 healthy controls (healthy control group) were enrolled in the study. Pulmonary function was measured in acute asthma exacerbation, post-treatment remission and healthy control group, respectively. Absorption assay (ELISA) measures the level of IL-9 in the induced sputum supernatant. Results The levels of IL-9 in induced sputum of patients with asthma attack were significantly higher than those of asthma patients (P <0.05). The levels of IL-9 in sputum of patients with asthma attack and remission were higher than those in healthy controls The difference was statistically significant (P <0.05). IL-9 levels in induced sputum of patients with asthma attack were positively correlated with neutrophil count and eosinophil count (r = 0.68,0.81, P <0.05, respectively), with forced expiratory volume in the first second accounted for The percentage of values (FEV%) was negatively correlated (r = -0.65, P <0.05). Conclusions IL-9 levels in induced sputum in patients with bronchial asthma are associated with airway inflammation and airflow limitation.