论文部分内容阅读
目的：研究川穹嗪（Tetramethylpyarajine，TMP）对家兔肠系膜微循环血管动态变化的影响，以求进一步明确TMP对微血管作用的机理。方法：采取显微录像静画步进法观测TMP对休克、炎症时的家兔肠系膜毛细血管血流速度、血流量、内径及毛细血管前括约肌变化的影响。用血管运动诱导法观测TMP对微血管的血管运动的作用。同时用两点光电法测量微血管运动血流量的变化。结果：1．平均动脉压维持在5．33kPa时TMP组使10μm以下毛细血管血流量一直维持在（1．24±0．15）10－3μl／s，而生理盐水组由（1．10±0．20）10－3μ／s下降到（0．60±0．30）10－3μl／s（P＜0．01），但两组内径与正常比皆无显著变化。2．平均动脉压维持在5．33kPa时TMP组使红细胞通过毛细血管前括约肌的时间明显短于酚妥拉明组。3．在肠系膜局部滴入去甲肾上腺素（NA）诱发出微动脉自律性血管收缩舒张运动，TMP可抑制由NA引起的血管运动，并呈量效依赖关系。抑制后的血管血流量增加，与微动脉并行的微静脉和淋巴管无变化。结论：TMP对血管作用的机理是抑制由NA诱发的微动脉及毛细血管前括约肌局部血管收缩舒张运动，疏通局部血流，增加毛细血管床的灌流量，而不是以往认为的扩张毛细血管床的口径。
Objective: To study the effect of Tetramethylpyarajine (TMP) on the dynamic changes of mesenteric microcirculation in rabbits in order to further clarify the mechanism of TMP on microvasculature. Methods: Microscopy video was used to observe the effects of TMP on capillary flow velocity, blood flow, inner diameter and precapillary sphincter changes in rabbits with shock and inflammation. The effect of TMP on blood vessel movement of microvessels was observed by vascular motion induction method. At the same time, the two-point electro-optical method was used to measure the change of microvascular blood flow. Results: 1. When the mean arterial pressure was maintained at 5.33 kPa, the TMP group kept the capillary blood flow below 10 μm at (1.24±0.15)10-3 μl/s, while the saline group consisted of (1.10±0.20). 10-3μ/s decreased to (0.60±0.30)10-3μl/s (P<0.01), but there was no significant change in inner diameter and normal ratio between the two groups. 2. When the mean arterial pressure was maintained at 5.33 kPa, the time for the TMP group to pass red blood cells through the precapillary sphincter was significantly shorter than that of the phentolamine group. 3. Local instillation of norepinephrine (NA) into the mesentery induces autonomic vasoconstriction and relaxation of the arterioles. TMP inhibits the vasomotor activity induced by NA and is dose-and effect-dependent. After the inhibition, the blood flow in the blood vessels increased, and there was no change in the venules and lymphatic vessels parallel to the microarteries. Conclusion: The mechanism of TMP’s effect on blood vessels is to inhibit the vasoconstriction and diastolic movement of the microarteries and precapillary sphincter induced by NA, to clear the local blood flow and increase the perfusion volume of the capillary bed, instead of the dilatation of the capillary bed previously thought. caliber.