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以78份不结球白菜栽培品种为试材,采用SSR分子标记技术,利用21对多态性对其进行多态性分析;并通过UPGMA法进行聚类分析。结果表明:该试验共产生了56个等位基因标记,每对引物的等位基因数2~4个;78份不结球白菜可分为三大类,类群I包括51份普通白菜、4份乌塌菜和3份菜心材料,遗传变异幅度较大,普通白菜来自南北方各地,反映了南北方栽培的普通白菜较近的亲缘关系;类群II包括2份菜心、3份普通白菜和5份苗用大白菜;类群III包含6份普通白菜、1份菜心和3份苗用大白菜。北方培育的苗用大白菜被分别聚在类群II和III,反映了其在选育过程中遗传背景发生了很大变异。该研究结果为不结球白菜遗传基础的拓宽和育种亲本的利用提供了科学依据。
Using 78 non-heading Chinese cabbage cultivars as test materials, SSR markers were used to analyze the polymorphism using 21 pairs of polymorphisms. Cluster analysis was performed by UPGMA. The results showed that there were altogether 56 alleles in this experiment, with 2 to 4 alleles per primer pair; 78 non-heading Chinese cabbage could be divided into three major groups, including 1 common cabbage and 4 Brassica juncea and cabbage core material 3, the genetic variation range, the common cabbage from all over the north and south, reflecting the cabbage cultivated in northern and southern regions closer genetic relationship; group II includes 2 parts of apricot, 3 parts of common cabbage And 5 parts of Chinese cabbage seedlings; group III contains 6 parts of common cabbage, 1 cabbage and 3 parts of Chinese cabbage seedlings. Miao Chinese cabbage cultivated in the north were clustered in groups II and III, respectively, reflecting the great genetic variation in the breeding process. The results provided the scientific basis for broadening the genetic basis of non-heading Chinese cabbage and utilizing the breeding parents.