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近年来,在饮用水管网中检测到耐受消毒剂的微生物,对于饮用水安全造成威胁.课题组由南方某城市自来水管网中分离出1株耐氯的产黏液分枝杆菌(Mycobacteria mucogenicum),并对其灭活特性和耐氯机制进行了研究.使用自由氯、一氯胺和二氧化氯对其进行消毒实验,测定CT值.99.9%灭活产黏液分枝杆菌时,自由氯的CT值为(76.25±47.55)mg·min·L-1,一氯胺为(1 396±382)mg·min·L-1,二氧化氯为(13.5±4.9)mg·min·L-1.采用透射电镜对产黏液分枝杆菌的消毒过程进行观察,发现消毒后细菌结构疏松,细胞器层次变得不清晰,核心肿胀溶解.产黏液分枝杆菌具有较好的疏水性,测定其表面疏水率为37.2%,远高于其他细菌,使得亲水性消毒剂不易进入细菌内部,是其耐受含氯消毒剂的原因之一.
In recent years, the detection of microorganisms resistant to disinfectants in the drinking water network has posed a threat to the safety of drinking water. A group of Mycobacteria mucogenicum (Mycobacteria mucogenicum ), And its inactivation characteristics and chlorine resistance mechanism were studied.Using free chlorine, monochloramine and chlorine dioxide to sterilize the experiment, determination of CT value .99.9% inactivation of Myxomycosis, free chlorine CT value was (76.25 ± 47.55) mg · min · L-1, monochloramine was (1 396 ± 382) mg · min · L-1 and chlorine dioxide was (13.5 ± 4.9) mg · min · L- 1. The transmission electron microscopy of Mycobacterium mucoid sterilization process was observed and found that after disinfection of bacteria loose structure, organelle level becomes unclear, swelling of the core swelling. Mycobacterium mucimmi has good hydrophobicity, the determination of the surface Hydrophobic rate of 37.2%, much higher than other bacteria, making hydrophilic disinfectant is not easy to enter the bacteria, is one of the reasons it is resistant to chlorine disinfectants.