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笔者利用乙型肝炎表面抗原(HBsAg)作为常见致病性病毒的替代物,进行了日光灭活试验观察。用反相间接血凝试验(RPHA)检测乙型肝炎表面抗原阳性血清,滴度在1:512以上共20份。每份取0.1ml 滴于无菌平皿内,涂成1cm~2面积,在日光下照晒一定时间后,以蒸馏水恢复原体积,同时设不照晒的对照组,两组同时用 RPHA 法检测 HBsAg 滴度,每批试验重复3次,求平均消毒率。夏季经4小时照晒20份标本除2份标本1:2阳性外,其余的标本均为阴性,其消毒率为98.4%,转阴率为100%;冬季经4小时照晒,只能降低2个滴度,其消毒率仅为75%。提示夏季的消毒效果是满
I use the hepatitis B surface antigen (HBsAg) as a common alternative pathogenic virus, the sun inactivation test observations. Hepatitis B surface antigen positive sera were detected by reverse phase indirect hemagglutination test (RPHA) with 20 titers above 1: 512. Each take 0.1ml drop in sterile petri dish, painted 1cm ~ 2 area, after sun exposure for a certain period of time, with distilled water to restore the original volume, while not sun control group, both by RPHA method HBsAg titers, each batch of experiments repeated 3 times, seek the average disinfection rate. In the summer, 20 specimens were sun-cured in 4 hours, except the 2 specimens of 1: 2 were positive, the rest were negative, the disinfection rate was 98.4%, the negative conversion rate was 100%; winter 4 hours after the sun, can only reduce 2 titers, the disinfection rate was only 75%. Tips summer disinfection is full