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伤寒Vi多糖菌苗在我国已试制成功,为了控制该茵苗的生产质量,必须建立一些实用的检定方法。由于Vi多精对酸水解有抗性和Vi单糖中存在氨基和按基基团,常用的比色法不适用于伤寒Vi多糖的测定。因此WHO规程中推荐使用火箭电泳法。用火箭电泳法检测多糖的浓度和分子大小国外已有
Typhoid Vi polysaccharide vaccine has been trial-produced in our country, in order to control the quality of the vaccine production, we must establish some practical test methods. Since Vi polygermanate is resistant to acid hydrolysis and amino groups and groups are present in Vi monosaccharides, commonly used colorimetric methods are not suitable for the determination of typhoid Vi polysaccharides. Therefore, rocket electrophoresis is recommended in the WHO protocol. Using rocket electrophoresis to detect polysaccharide concentration and molecular size have been abroad